96-7 DNA Polymerase
Heat-resistant strand displacement DNA polymerase| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
96-7 DNA Polymerase |
319-07301 |
1,600 units |
Product Description
This product is an enzyme having heat-resistant property and a strand-displacement type DNA polymerase activity, which synthesizes a new DNA strand while dissociating the hydrogen bond of the double stranded template DNA by itself. Since the strand-displacement DNA polymerase does not require dissociation of double-stranded DNA by its characteristics, DNA can be synthesized at a constant temperature, and the synthesis is not inhibited by the secondary structure of DNA.
Characteristics
- 5'→3' DNA polymerase activity and strand-displacement activity.
- DNA synthesis can be performed at a constant temperature.
- Suitable for synthesis of DNA strands having high GC content
- Not affected by inhibition due to the secondary structure of DNA
- High purity/high quality control
Applications
- Application using the strand displacement activity (Isothermal and Chimeric Primer-initiated Amplification and the like)
Optimum reaction temperature |
50°C - 55°C |
|---|---|
Deactivation temperature*1 |
70°C, for 5 min. |
*1 Deactivation temperature when the stock enzyme solution is directly heat-denatured.
| Concentration | 8 units/µl |
|---|---|
| Unit Definition | One unit is defined as the enzyme activity incorporating 10 nmoles of deoxynucleotide into acid-insoluble precipitates at 55°C in 30 min using activated calf thymus DNA as a primer/template. |
| Storage Conditions | 10 mmol/l Tris-HCl (pH 7.5), 1 mmol/l DTT, 0.1% Tween 20, 0.1 mmol/l EDTA, 50% Glycerol |
| Reaction Conditions | 1 x 96-7 Reaction Buffer |
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Product Components
| Component | Quantity | Storage | Description |
|---|---|---|---|
| 96-7 DNA Polymerase (8 units/µl) |
200 µl x 1 | -20°C | - |
| 10 x 96-7 Reaction Buffer (95 mmol/l Mg2+) | 500 µl x 1 | -20°C | - |
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Usage
Usage example: Application for LAMP method
3.0% Agarose 21
TAE gel electrophoresis,
EtBr staining
M：
Gene Ladder Wide 1 (Code No. 313-06961)
1： Strand displacement type DNA polymerase
derived from the Geobacillus genus.
2： Csa DNA Polymerase
3： 96-7 DNA Polymerase
Candidatus Liberibacter asiaticus DNA |
1 x 106 copies |
FIP* |
40 pmol |
BIP* |
40 pmol |
F3 Primer* |
5 pmol |
B3 Primer* |
5 pmol |
Loop Primer F* |
20 pmol |
Loop Primer B* |
20 pmol |
dNTPs Mixture |
2.0 mM each |
10 x 96-7 Reaction Buffer |
2.5 µl |
96-7 DNA Polymerase |
8 units |
Total |
25 µl |
| 60°C | 60 min. |
|---|
| * Primer Sequence | |
| FIP: 5'-GCATGCCGAGGATCAATGCCTTGCTTAAAGAGCGTGCTACG-3' BIP: 5'-TATGCCTAATGGCACGGGGGTAAGCTTCATCCGCCTTCGA-3' F3 Primer: 5'-TGGGTTAAGTGATGCTGTGG-3' B3 Primer: 5'-CAACAATATCAGCCCCTGCT-3' Loop Primer F: 5'-TCTCAACTGTTTCATCAAACCTAGC-3' Loop Primer B: 5'- CGTGGCGGTTTTTGCTACA-3' |
|
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Usage example: Evaluation of thermal resistance and optimum reaction temperature by RCA method
M13mp18 single strand DNA |
20 ng |
Universal primer |
50 nM |
dNTPs Mixture |
0.25 mM each |
Tris-HCl (pH 8.8 at 25°C) |
20 mM |
KCl |
10 mM |
(NH4)2SO4 |
10 mM |
MgSO4 |
2 mM |
Tween 20 |
0.1% |
DNA Polymerase |
8 units |
Total |
20 µl |
| for 30 min | at respective temperatures |
|---|
 |
0.7% Agarose S /
TAE gel electrophoresis, |
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Data Sheets
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
- The LAMP primer set for detecting Candidatus Liberibacter asiaticus was developed at the National Agricultural Research Center for the Kyushu Okinawa Region, in “Development of preventive technologies for hard to control citrus greening disease” of Research Project Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries.
License
- The patent for the LAMP method is owned by EIKEN Chemical Co., Ltd..
Related Products
Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
