Gene Taq
Taq DNA polymerase| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
Gene Taq |
312-02874 |
50 units |
||
Gene Taq |
318-02871 |
250 units |
||
Gene Taq |
314-02873 |
250 units x 4 |
Product Description
Gene Taq is a heat-resistant DNA polymerase which is obtained by cloning the DNA polymerase gene of Thermus aquaticus YT1, modifying the gene, expressing it in Escherichia coli, and separating and purifying.
Gene Taq is suitable for PCR. Also, having a terminal transferase activity, the PCR product obtained can be used for TA cloning.
Since the N-terminal of Taq DNA polymerase is partially deleted, the 5'-3' exonuclease activity is lost.
Also, the amplification efficiency for DNA fragments of not more than 1 kbp is especially high.
Applications
- PCR
| Molecular Weight | 68 kDa |
|---|---|
| Concentration | 5 units/μl |
| Storage Conditions | 20 mmol/l Tris-HCl (pH 8.0)， 100 mmol/l KCl, 0.1 mmol/l EDTA, 0.5% Tween 20, 1 mmol/l DTT, 50% Glycerol |
| Unit Definition | One unit is defined as the enzyme activity incorporating 10 nmoles of deoxynucleotide into acid-insoluble precipitates at 74°C for 30 min using activated calf thymus DNA as a primer/template. |
| Purity | No change was observed in the agarose gel electrophoresis pattern of DNA after reacting 10 units of this enzyme and 1 µg of λ/Hind III digest at 74°C for 1 hr. |
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Product Components
| Component | Quantity | Storage | Description |
|---|---|---|---|
| Gene Taq | 50 units x 1 | -20°C | |
| dNTP Mixture (2.5 mmol/l each) | 160 μl x 1 | -20°C | |
| 10 x Gene Taq Universal Buffer (15 mmol/l Mg2+) | 1 ml x 1 | -20°C |
| Component | Quantity | Storage | Description |
|---|---|---|---|
| Gene Taq | 250 units x 1 | -20°C | |
| dNTP Mixture (2.5 mmol/l each) | 800 μl x 1 | -20°C | |
| 10 x Gene Taq Universal Buffer (15 mmol/l Mg2+) | 1 ml x 1 | -20°C |
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Data Sheets
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
Related Products
| Product | Characteristics | 5' → 3' exonuclease activity | terminal transferase activity |
|---|---|---|---|
| Gene Taq | The amplification efficiency of DNA fragments not more than 1 kbp is especially high. | － |
＋ |
| Gene Taq NT | Gene Taq NT has the same functions as natural Taq DNA polymerase. | ＋ |
＋ |
| Gene Taq FP | Gene Taq FP obtained by further purifying Gene Taq to a higher degree of purity and by controlling the contamination by the host-derived DNA as much as possible. It is suitable for RAPD PCR which is affected by contamination by the genomic DNA. |
－ |
＋ |
Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
