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Product Description

Unit definition

One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.

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Product Components

Restriction Endonuclease (unit packaging)

Component	Quantity	Storage	Description
Restriction Endonuclease	(unit packaging) x 1	-20°C	Please go to the page in Japanese.
10X supplied buffer	1 ml x 1	-20°C	Supplied buffer is 10 times concentration.

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Usage

10X buffer is supplied, respectively.

10X supplied buffer	Compositions	Enzyme
10X L buffer	100 mmol/l Tris-HCl (pH 7.9 at 25°C) 100 mmol/l MgCl2 10 mmol/l DTT	Alw44 I*1, Apa I , Kpn I , Mbo II , Nar I , Nci I , Nsp V , Sac I , Sac II
10X M buffer	500 mmol/l NaCl 100 mmol/l Tris-HCl (pH 7.9 at 25°C) 100 mmol/l MgCl2 10 mmol/l DTT	Acc II , Age I , Alu I , Ava I , Ava II , Axy I , Dra I , EcoO109 I , EcoR II , EcoT38 I , Fok I , Hae II , Hae III , Hinc II , Msp I , Nhe I , Pvu II , Rsa I , Sau3A I , Sau96 I , Sfi I , Spe I , Stu I , Xba I
10X H buffer	1,000 mmol/l NaCl 500 mmol/l Tris-HCl ( pH 7.9 at 25°C) 100 mmol/l MgCl2 10 mmol/l DTT	Ase I , Bcl I , Bgl I , Bgl II , BstE II , BstX I , EcoR I , EcoR V , Hinf I , Mlu I , Nco I , Nde I , Not I *2, Nsi I , Pst I , Sal I , ScrF I , Sph I , Sty I , Swa I *1, Xho I
10X A buffer	500 mmol/l Potassium acetate 200 mmol/l Tris-acetate (pH 7.9 at 25°C) 100 mmol/l Magnesium acetate 10 mmol/l DTT	Acc I , Afl II , Bsp1286 I , Fsp I , Sma I , Taq I
10X B buffer	1,000 mmol/l NaCl 100 mmol/l Tris-HCl (pH 8.5 at 25°C) 100 mmol/l MgCl2 10 mmol/l DTT	Acc III *1, Acy I , BamH I , Bsm I , BssH II , Hha I , Hind III , Ssp I
10X Bal I buffer	200 mmol/l Tris-HCl (pH 8.5) 70 mmol/l MgCl2 10 mmol/l DTT	Bal I
10X Hpa I buffer	1,000 mmol/l KCl 100 mmol/l Tris-HCl (pH 7.5) 70 mmol/l MgCl2 10 mmol/l DTT	Hpa I
10X Nde II buffer	1,500 mmol/l NaCl 1,000 mmol/l Tris-HCl (pH 7.5) 100 mmol/l MgCl2 10 mmol/l DTT	Nde II
10X Nru I buffer	1,500 mmol/l KCl 60 mmol/l Tris-HCl (pH 7.5) 60 mmol/l MgCl2 10 mmol/l DTT	Nru I
10X Sca I buffer	1,250 mmol/l NaCl 60 mmol/l Tris-HCl (pH 7.5) 60 mmol/l MgCl2 10 mmol/l DTT	Sca I
1 mg/ml BSA	1 mg/ml BSA	Alw44 I, Swa I , Acc III *1 a final concentration of 0.1 mg/ml BSA.
0.1% Triton X-100	0.1% Triton X-100	Not I *2 a final concentration of 0.01% Triton X-100.

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Data Sheets

SDS(Safety Data Sheet)

• Please go to the page in Japanese.

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Related Information

Note

• This product is for research use only.

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