Agarose 21
Agarose for Gel Electrophoresis| Product Name | Code No. | Size | Price | Note (Packaging) |
|---|---|---|---|---|
Agarose 21 |
311-03243 |
3 g |
Stick type (Plastic Bag) | |
Agarose 21 |
315-03241 |
3 g x 25 |
Stick type (Plastic Bag) | |
Agarose 21 |
313-03242 |
25 g |
Plastic Bottle | |
Agarose 21 |
319-03244 |
100 g |
Plastic Bottle | |
Agarose 21 |
- |
500 g |
Plastic Bottle | |
Agarose 21 |
- |
1 kg |
Plastic Bottle |
Product Description
Agarose 21 is developed for separating nucleic acid of 1,000bp or less and is suitable for electrophoresing short chain DNA such as PCR products and restriction endonuclease fragments, and RNA.
This product is provided as the stick type and the bottle type, and in the case of the stick type, there is no need of weighing agarose powder, and 100ml of 3% agarose gel can be prepared from one stick (3g).
Further, the handling of the gel during the manipulation is easy because this product has a superior solubility and high gel strength.
Features
・low EEO・DNase free, RNase free・Recommended Gel Concentration: 3 % (2 - 5 %)・Nucleic Acid Fragment Separation Range: 10 bp - 1000 bp
Applications
- Gel Electrophoresis for separation of DNA fragments
- Recovered DNA*1
- Blotting
*1 Recovered DNA from preparative Agarose gels is checked that it can be used for downstream applications (cleavage by restriction endonucleases, ligation by T4 DNA ligase, E. coli transformation by ligated DNA.)
| Gel strength | ≥ 800 g/cm2 (3%) |
|---|---|
| Melting point | ≤ 85°C (3%) |
| Gelling temperature | 34 - 38°C (3%) |
| Sulfate content (SO4) | ≤ 0.1% |
| Water content | ≤ 10% |
| Electroendosmosis (-Mr) | ≤ 0.1 (Low EEO) |
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Usage
Recommended Gel Concentration
- 3 % gel in TAE buffer or TBE buffer
(In the case of the stick type, there is no need of weighing agarose powder, and 100ml of 3% agarose gel can be prepared from one stick (3g).)
Recommended Gel Thickness
- 3 mm
(Clear electrophoresis patterns can be obtained by limiting the gel thickness to about 3mm.)
Usage Note
At a gel concentration higher than 5%, the solution becomes highly viscous and may be difficult to handle. When melting, cover the container with a plastic wrap to prevent buffer evaporation and open a pinhole.
Separation of DNA markers in Agarose 21
Data 1: Comparison with Agarose X
run at 60V for 4hr. in TAE Buffer
3% Agarose 21, 3% Agarose X
12.5 cm x 10 cm x 3 mm
Lanes (0.5 µg/lane)
・M4 : Marker 4 (φX174/Hae III digest)
・M5 : Marker 5 (φX174/Hinc II digest)
・M9 : Marker 9 (φX174/Hinf I digest)
・M10 : Marker 10 (pBR322/Msp I digest)
Data 2: Test for the minimum detectable amount of DNA
run at 60V for 4hr. in TAE Buffer
3% Agarose 21 (12.5 cm x 15 cm x 3 mm)
0.05g - 2.0g Marker 4 (φX174/ Hae III digest)
Data 3: Electrophoresis in Mini-gel (Mupid® )
run at 50V for 80min. in TAE Buffer
3% Agarose 21
Lane 1: 0.1 µg DNA Marker / lane
Lane 2: 0.5 µg DNA Marker / lane
M4 : Marker 4 (φX174/Hae III digest)
M5 : Marker 5 (φX174/Hinc II digest)
M9 : Marker 9 (φX174/Hinf I digest)
M10 : Marker 10 (pBR322/Msp I digest)
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Data Sheets
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
Related Products
- Agarose S (Gel range: 0.5 - 2%)
- Agarose S Tablet (0.5g of Agarose S/tablet)
- Agarose XP (Gel range: 1 - 4%)
- Agarose H (Gel range: 0.2 - 1%)
Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
