Top> Reagents for research> Agarose X

Product Description

Agarose X is developed for separating nucleic acid of 1,000 bp or less and is suitable for electrophoresing short chain DNA such as PCR products and restriction endonuclease fragments, and RNA. Further, the handling of the gel during the manipulation is easy because this product has a superior solubility and high gel strength.

Features

・low EEO・DNase free, RNase free・Recommended Gel Concentration:　3 % (2 - 6 %)・Nucleic Acid Fragment Separation Range:　10 bp - 1000 bp

Applications

• Gel Electrophoresis for separation of DNA fragments
• Recovered DNA^*1
• Blotting

*1 Recovered DNA from preparative Agarose gels is checked that it can be used for downstream applications (cleavage by restriction endonucleases, ligation by T4 DNA ligase, E. coli transformation by ligated DNA.)

Specification

Gel strength	≥ 1,200 g/cm2 (4%)
Melting point	≤ 92 °C (4%)
Gelling temperature	31 - 39°C (4%)
Sulfate content (SO4)	≤ 0.15%
Water content	≤ 10%
Electroendosmosis (-Mr)	0.06 - 0.14 (Low EEO)

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Usage

Recommended Gel Concentration

• 3 % gel in TAE buffer or TBE buffer

Recommended Gel Thickness

• 3 mm
  (Clear electrophoresis patterns can be obtained by limiting the gel thickness to about 3mm.)

Usage Note

At a gel concentration higher than 6%, the solution becomes highly viscous and may be difficult to handle. When melting, cover the container with a plastic wrap to prevent buffer evaporation and open a pinhole. The solution may be slightly yellowish but there is no problem of the quality.

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Data Sheets

SDS(Safety Data Sheet)

• Please go to the page in Japanese.

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Related Information

Note

• This product is for research use only.

Related Products

• Agarose S (Gel range: 0.5 - 2%)
• Agarose S Tablet (0.5g of Agarose S/tablet)
• Agarose XP (Gel range: 1 - 4%)
• Agarose H (Gel range: 0.2 - 1%)

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