ISOGEN and ISOGEN-LS
Isolation of total RNA| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
ISOGEN |
315-02504 |
10 ml |
(phenol formulations) | |
ISOGEN |
317-02503 |
50 ml |
(phenol formulations) | |
ISOGEN |
311-02501 |
100 ml |
(phenol formulations) | |
ISOGEN-LS |
317-02623 |
10 ml
|
(phenol formulations) | |
ISOGEN-LS |
311-02621 |
100 ml |
(phenol formulations) |
Product Description
ISOGEN and ISOGEN-LS are reagents for RNA extraction from humans, animals, plants and bacteria. The liquid phase separation method is used, and DNA and protein can be isolated from a single sample. Since RNA, DNA and protein can be isolated through a series of manipulations, it is very effective for analyzing precious samples, and the simplicity of the manipulations allows a large number of samples to be handled.
ISOGEN and ISOGEN-LS are homogenous liquids containing phenol and guanidine thiocyanate, with ISOGEN-LS containing more phenol than ISOGEN. ISOGEN is used for the samples of tissues and cultured cells, and ISOGEN-LS is effectively used for samples of large volume such as blood (> 100 µl when processed in a 1.5 ml plastic tube) and liquid samples that are difficult to concentrate.
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Product Components
| Component | Quantity | Storage | Description |
|---|---|---|---|
| ISOGEN | 100 ml x 1 | +4°C | blue solution (phenol formulations) |
| Component | Quantity | Storage | Description |
|---|---|---|---|
| ISOGEN-LS | 100 ml x 1 | +4°C | red solution (phenol formulations) |
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Usage
Precautions
- This product is a reagent for research and cannot be used for other objectives. Also, this product should be handled only by persons having a basic knowledge about reagents.
- ISOGEN and ISOGEN-LS are deleterious substances not for medical use (phenol formulations), and should be handled with great care.
- This product is a reagent for research and cannot be used for other objectives. Also, this product should be handled only by persons having a basic knowledge about reagents.
- When using, wear appropriate protective gear (gloves, goggles, etc.).
- If the product enters eye or adheres to skin, wash with large amounts of water for at least 15 min and consult a doctor.
- Handle this product in accordance with the descriptions in the manual.
- We are not responsible for problems caused if this product is not handled in accordance with the manual.
Plotocol
ISOGEN and ISOGEN-LS are homogenous liquids containing phenol and guanidine thiocyanate, with ISOGEN-LS containing more phenol than ISOGEN. ISOGEN is used for the samples of tissues and cultured cells, and ISOGEN-LS is effectively used for samples of large volume such as blood (> 100 µl when processed in a 1.5 ml plastic tube) and liquid samples that are difficult to concentrate.
ISOGEN or ISOGEN-LS is added to a sample, and after dissolving or homogenizing, chloroform is added to the mixture and centrifuged. The homogenate is separated into 3 phases: an aqueous phase, an organic phase and an intermediate phase. In the aqueous phase, only RNA is present, and DNA and protein are present in the intermediate phase and the lower phase. The aqueous phase is collected, and RNA is precipitated by adding isopropanol. DNA is precipitated by adding ethanol to the residual intermediate phase and organic phase, and protein is then precipitated by adding isopropanol. In this way, RNA, DNA and protein can be sequentially isolated.
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Data Sheets
Technical Data Sheet
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
- Licensed by Molecular Research Center Inc.
Reference
- Chomczynski, P. and Sacchi, N.: Anal.Biochem., 162, 156 （1987）
- Chomczynski, P.: BioTechniques, 15, 532 （1993）
- Chomczynski, P., Bowser-Finn, R. and Sabatini, N.: The Jounal of NIH Research, 6,83 （1994）
- Chomczynski, P. and Mackey, K., BioTechniques, 19, 942-945 （1995）
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- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
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