Ligation-Convenience Kit
2X Ligation Mix| Product Name | Code No. | Size | Price | Note |
|---|---|---|---|---|
Ligation-Convenience Kit |
315-05963 |
10 ligations |
||
Ligation-Convenience Kit |
319-05961 |
100 ligations |
Product Description
The first important step of gene cloning is to ligate the target gene to a vector DNA. The efficiency of the ligation reaction varies depending on the shape of the DNA terminal, and until now the ligation reaction has needed to be optimized for each reaction. Further, the ligation reaction requires 1-16 hours using a conventional T4 DNA ligase, and it has been difficult to obtain high transformation efficiency. The Ligation-Convenience Kit can solve these problems and provide highly efficient ligation in a short period of time.
Characteristics
- This product is a kit for performing DNA ligation rapidly and simply.
- The 2X Ligation Mix contains all the components required for DNA ligation such as the reaction buffer, ATP, DTT, T4 DNA Ligase, etc. The DNA ligation reaction can be performed by adding an equal volume of 2X Ligation Mix to the DNA solution.
- By using this kit, the ligation reaction can be performed in 5-30 min regardless of the shape of the DNA terminal. Further, the DNA solution in which the ligation reaction has been completed can be used for transformation and in vitro packaging as-is.
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Product Components
| Component | Quantity | Storage | Description |
|---|---|---|---|
| 2X Ligation Mix |
100 μl x 1 | -20°C | - |
| Component | Quantity | Storage | Description |
|---|---|---|---|
| 2X Ligation Mix |
250 μl x 4 | -20°C | - |
Note
- 10 μl of 2X Ligation is used in a total reaction volume of 20 μl.
- At the time of use, thaw completely on ice and mix well by pipetting.
- No reduction in reaction efficiency was found in up to 50 cycles of freezing and thawing.
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Usage
Protocol
DNA solution by mixing a vector-DNA and an insert-DNA (10 µl)
↓←10 µl, 2X Ligation Mix
↓mix
↓ligation reaction at 16°C for 5-30 min.
Transformation
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Data Sheets
Technical Data Sheet
SDS(Safety Data Sheet)
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Related Information
Note
- This product is for research use only.
Related Products
Contact us
- Distributor
- FUJIFILM Wako Pure Chemical Corporation
- Produced by
- NIPPON GENE CO., LTD.
- E-mail：Web Contact Form
